No heating or other preparation of the ladder is required. Along with protein samples of interest, simply load one of the lanes on the gel with 4 µL of ScanLater Western Blot Protein Ladder. The ScanLater Western Blot workflow follows standard gel loading and blotting methods up to the secondary antibody incubation step. The linear relationship between the molecular weight of the biotinylated ladder proteins and their migration distance on the gel is examined in Figure 2 in comparison with a reference standard. They are also seen as dark bands when the ladder is read with the Scanlater Western Blot Detection System. These blue bands are seen on the electrophoresis gel and on the blot after transfer. In addition, there are three visible pre-stained marker proteins at 18, 31, and 70 kD (Figure 1, right). They are detected by incubating the blot with Europium-labeled streptavidin and they show as light colored bands when read with the ScanLater Western Blot Detection Cartridge (Figure 1, left). The ScanLater Western Blot Protein Ladder consists of seven biotinylated recombinant proteins at 10, 20, 40, 50, 80, 100 and 140 kD. The blot is read with the ScanLater ™ Western Blot Detection Cartridge on the SpectraMax ® i3 or SpectraMax ® Paradigm ® Multi-Mode Microplate Readers. Detection of the biotinylated proteins is enabled by adding Europium-labeled streptavidin to the blot at the secondary antibody step. The S canLater Western Blot Protein Ladder consists of seven biotinylated recombinant proteins as well as three prestained marker proteins. The primary applications of this protein ladder include the estimation of protein molecular weight, the visualization of gel electrophoresis, and the evaluation of the transfer process from gel to blot. The ScanLater ™ Western Blot Protein Ladder is an essential component of the ScanLater ™ Western Blot Detection System. Molecular weights of detected proteins can be estimated.A single set of standards contains markers for both visual gel and blot alignment plus TRF detection.For most researchers, we generally recommend:Ī) ab116027 for proteins between 10 and 180 kDaī) ab116028 for larger proteins up to 245 kDaĬ) ab116029 for smaller proteins down to 3.5 kDaĭ) ab116029 or ab234592 for studying smaller and larger proteins at the same time.Application Note Estimation of Protein Molecular Weights with ScanLater Western Blot Protein Ladder When choosing the right pre-stained protein ladder, the most important choice is based on the size of your protein of interest. Prestained ladders also can use highlight bands of a different color, to make it easier to identify different size bands. Protein ladders are most convenient to use when they are supplied ready to use, with no heating, dilution, or addition of reducing agent required before loading onto the gel. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. However, an unstained protein ladder can only be visualized following staining with Coomassie or a similar non-specific protein stain. Using an unstained protein ladder is very useful when you need to accurately calculate the size of your protein. calculate the approximate size of your protein by overlaying an image of the membrane with the ladder with the image generated by antibody staining. verify transfer efficiency between gel and membrane (PVDF, nylon, or nitrocellulose) monitor protein separation during SDS-polyacrylamide gel electrophoresis Using a prestained protein ladder when running a western blot helps when you need to:
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The game was received well by fans for its "dedication needed to master". This latest iteration of the 30-year RPG Maker series has a variety of new and overhauled features that automate parts of the gamedev process, so you can focus on the fun. RPG Maker 2 contains the developers' pre-created sample game called FuMa to showcase an example of a fully completed game built with the software. RPG MAKER UNITE enables creators of all skill levels to build classic 2D RPG games in Unity without writing code. Users also have the ability to create their own music tracks if needed. The musical score of RPG Maker 2 contains a small collection of samples. It is ideal for hobby games, personal projects, school assignments, etc. The game also includes a real-time system that allowed changes in time and weather, letting the creator design time-specific events. Create your game by dragging and dropping items into your world. Like other game-making programs, RPG Maker 2 gives the ability to create different events, drive cutscenes, construct boss battles, and add various effects (activities' execution is tied to pre-generated scripts, as in most programming languages). The PlayStation 2 USB port also allows the use of a USB keyboard, speeding up the process of writing text. Instead, RPG Maker 2 uses Picture Paradise, software that allows the user to incorporate digital photos into the game. Find game assets tagged RPG Maker and Sprites like Free - Pixel Art Asset Pack - Top down RPG - 16x16 Dungeon Crawler Sprites, Simple Dungeon Crawler 16x16 Pixel Art Asset Pack, Isometric Dungeon Crawler - monogon, RPG Nature Tileset - Seasons, Isometric Cyberpunk City - monogon on itch. The game uses super-deformed character models outside of battle, with somewhat more realistic but still stylized models in battle.īecause RPG Maker 2 used 3D graphics instead of 2D, programs like the Anime Maker for RPG Maker were not included in RPG Maker 2. It was also the first one that allowed players to move in any direction by manipulating the analog stick, but the graphical quality of the game suffered during movement, providing a "blur" effect. This makes Figuro very suitable for beginner and. Figuro is used by game developers, designers, hobbyists, students and more Figuro offers powerful 3D software yet focusses on simplicity. You can use Figuro to make 3D models for games, prototypes, architecture, art and so on. RPG Maker 2 was the first console RPG Maker to feature full 3D graphics. Figuro is a free online 3D modeling application for everyone. The game lets players write their own stories and uses 3D models. RPG Maker is where you overlay an image that youve edited on t. ・You may modify some of the assets (but not redistribute them) for your own game use only.RPG Maker 2 ( RPGツクール5, RPG Tsukūru 5) is the third PlayStation version of the RPG Maker series, following the original RPG Maker (and the Japan-only RPG Tsukūru 4), the first version of the series to be released for the PlayStation 2 and the overall fifth installment for home consoles. In this video tutorial, Ill show you have to add parallax mapping to your RPG Maker MZ Game. ・OK to be used in Commercial projects made in RPG Maker ・This pack is for use in RPG Maker Series or the engine of your choice. Original size material for face graphics. Plains, wastelands, oceans, snowfields, etc. *Some of the asssets are not up to standard, so they are suitable for intermediate to advanced users.ĥ6 battle backgrounds in 2 sizes (320x240, 640x480)ġ35 face graphics (without facial expressions)Ģ5 types of tilesets. The collection can be used not only for simulation RPGs, but also for a variety of games depending on your ideas! Please take a look at the nostalgic yet high quality dot-picture assets! The collection includes tilesets, characters, animations, and other graphic materials as much as possible as they were at that time! Graphic materials for "Simulation RPG Maker" released in 1998 are now available as a collection of materials for RPG Maker MZ! After cutting off all of them and forming a seven-piece tangram puzzle, reassemble them to make sure you have done it correctly.Ĭongratulations! You have made your first tangram square. Be accurate, and don’t cut too far into pieces. Now that you have measured and marked the tangram template, it is time to cut out the tangram shapes.When you start to cut out the tangram shapes that have been lettered, the tangram shapes that haven’t been lettered will form independently. Keep in mind that each side of a square is even.Here are the measurements for each letter: As seen in the image, each side has been lettered. Measurements are crucial for the tangram puzzle to work.Use your ruler and pencil to measure and trace out a 16 centimeter square on the cardboard.However, it is believed that the name itself must have existed before Christ.Ī tangram activity brings a lot of fun, but we will double it with the instructions on how to make tangram shapes: Here comes the ancient but still famous and full of a fun game: Tangram! This old Chinese puzzle has kept its popularity for hundreds of years now.įact: Although it is still not sure who invented it, the history of tangrams is traced to a book in the nineteenth century and an old painting of two Chinese people playing tangram that dates back to the eighteenth century. You can find hundreds of fun telling time games and learning games for toddlers and kindergartners.ĭIY learning games for kindergarten are both unique and fun! Take a look at these interesting games and make your pick. It was developed by academicians and pedagogues especially to support children’s cognitive development. MentalUP is one of the best apps for kindergarten students. Gamified learning is a proven learning approach that is widely recommended by scientists. In addition, to memorize these words, we also recommend the 3rd-grade students learn how to run the online features of the website, which will improve the efficiency of studying the Fry words list. He invented this list because he found them appearing often in children’s books. The Dolch sight word list came from an educator in the 1930s known as Dr. Fry 300 is a prerequisite to learning other Fry words in higher grades. Are Dolch and Fry Sight Words the Same These sight word lists are not the same, but sometimes you will hear them being used interchangeably. No matter what they studied before, grade 3 students should master all of them. The third 100 Fry words plus the first 100 and second 100 words are called Fry 300, which are the essential words of the Fry sight list. The Dolch Sight Words list is the most commonly used set of sight words, and it is the one I use. The third 100 Fry words are the right content for children who finish Dolch words and turn to more extensive sight vocabulary. That’s when I turn to the old traditional Dolch Sight Word List and the Fry Lists of sight words to find out the Kindergarten First 100 Sight Words I need to teach. He based the list of the most common words in children’s books during the 1930s and. It contains 220 ‘service words’ and 95 high-frequency words. The list of Dolch sight words is the most commonly used list. In this year, the original Dolch students have to switch to Fry words sooner or later. Common Core sight words, a new variation that combines Dolch and Fry sight words into new combinations of lists. In grade 1 and grade 2, some students stick to Dolch words. For example, selecting the Dolch Sight Words Kindergarten and 1st Grade lists uses those 93 words for your Flash Cards. Under Dolch Sight Words or Fry Sight Words or Top 150 Written Words, check the boxes corresponding to any sight words lists you want included in your Flash Cards. Comparing the first 300 Fry words (also called Fry 300) with Dolch words will get a high ratio overlapping. First, select the sight word list (s) you wish to use. So, the 3rd grade is the particular year to study Fry words. The Dolch sight words list focuses on reading from kindergarten to 2nd grade, and the Fry sight words list focuses on reading from 3rd to 9th grade. Kindergarten Sight Words made simple and fun Free kindergarten sight word lists, printables, worksheets, games, assessments, and activities. The Control Center has a user-friendly interface that makes it easy to view live footage, manage recordings, and add devices. This includes datasheet, user guide, End User License Agreement EULA, software and license upgrade guide, and others. Trusted Windows (PC) download Avigilon Control Center Player 5.10.4.6. Best Practice: Use the same version of ACC Server and other ACC components on one server, multi-server Site cluster, and viewing stations. High quality media solution for large libraries. NOTE: AVE stands for Avigilon Native Video Export. The Avigilon Control Center is a free download that allows you to manage your security cameras and access footage from anywhere. This document presents ACC 7 features (ACC 7.0 to 7.6). The Avigilon Control Center Player is included with the Avigilon Control Center Client software but can also be downloaded from the Avigilon website. In the "Format" dropdown menu, select "Native" to export video as an AVE file. To export video in Avigilon Control Center Player, select the "Export" icon in the "Export" tab, choose the format from the "Format" dropdown menu, select the camera video you would like to export, enter the time range, set your desired options, and click Start Export. You can remove the video from a camera that is being displayed by right-clicking the image panel and selecting "Close" or clicking the "X" icon inside the image panel. What is the Avigilon Control Center Player. If the AVE file stores video recorded from multiple cameras, each video is displayed by default. Site cluster refers to a multi-server ACC site.When opening an AVE file in Avigilon Control Center Player, the video is immediately displayed in the View. Copy the license keys for this recorder if it is NOT part of a site cluster.You will use these installers again after installing the operating system.ĭo not use the Windows OS disk because its contents will be deleted during the upgrade process. Go to the download location and copy the installer file to the storage volume (typically D:) on your recorder or your external storage device.If you do not have access to the article, contact your integrator for licensing details. Tip: If you want exported video to display the same time as the ACC Server, export the video from a client that is in the same time zone as the server. The Avigilon Control Center is a free download that allows you to manage your security cameras and access footage from anywhere. The exported video displays 2:00AM PST when it is played back in the ACC Player. When the application first opens, you will be prompted to open an AVE or AVK file. A video recorded at 10:00AM UTC is exported from an ACC Client in the Pacific time zone. In Windows, select All Programs or All Apps > Avigilon > Avigilon Control Center Player > Avigilon Control Center Player. The Avigilon Player displays video in image panels, and allows you to control their playback through the Timeline. Using the link given below and proceeding to the developers website in order to download Avigilon Control Center Player was possible when we last checked. The Avigilon Player is the video player for Avigilon Native Video Export (AVE) files and Avigilon Backup (AVK) files. In the results, locate the ACC Server release to download and click the EXE File Download button to initiate the download.įor more information about upgrading to ACC 7 and obtaining the required installers, see ACC 7 Software Upgrade Guide ( /support/software/acc7) and Knowledge article, How to Upgrade Servers to ACC 7 ( article 10085) in the Avigilon Support Community. The Player can be opened in any of the following ways: Double-click the shortcut icon on the desktop. Thank you for using our software library.In the Category field, select the version of the ACC software to download ( ACC 7, ACC 6 or ACC 5).Log in to the Avigilon Support Community: .Ĭlick Software Downloadsin the top menu bar.Downloading the Latest Release of the Installed ACC Versionįrom a computer connected to the internet, download the installer for the latest release of the ACC version that is installed on your system. Avigilon Control Center Client is a great tool for managing and monitoring your Avigilon High Definition Surveillance System. The most recent release as of writing is 3.2. An easy way to get to the latest version is to simply Google 'Waifu Sex Simulator' and click the latest link from. ( that mean you can import mikumiku models and scene/motion in waifus ex simulator, that boost the amount with even more ) and yes, they are hundreds of scenes. Installation Installing the game is fairly straightforward, but may take a long time depending on your internet connection speed. It is possible to create character motions by built-in MotionEditor. At least I can't find them.įor example, there are some nice bodies in this picture on this board. also, for who doesnt know, most of characters from mikumikudance are compatible with mocumocudance. MocuMocuVRM - 'MocuMocuVRM' is an application to view VRM character models and motions by SteamVR. In XNALARA there a lot of nude "hunk / muscle" models with great textures, but no young and slim boys. Waifu Sex Simulator v3.9 Lewd Fraggy Dive into the world of anime and hentai, choose your favorite character from a pool of more than 1,000 models coming from the most famous animes, games, and tv shows. If you include all the models that can be imported from sites like DeviantArt, you have more virtual waifus than even the horniest weeb could fuck in his entire lifetime. The game has 100’s of different models to choose from. I would like to have nude (they do not have to have genitals) models of slim and athletic teen boys / young males.Īs MMD is very famous in Japan, there are tons of nude female teens, but just very few boy models (with very low detail textures). Waifu Sex Simulator is a VR porn game created by Lewd Fraggy. They can choose from several 3D models which can be customized with personalized skin and clothing colour. In Waifu Sex Simulator, players are able to create a wide range of custom characters based on their preferences. Experience genuine, unrestricted NSFW AI interactions with AI characters - Your AI Girlfriend. The game has generated a lot of attention due to its graphical quality, realistic animation, and detailed simulation elements. Dive into NSFW Character AI chats without filters. Shes your fathers friend and your landlady. There are basically three sourcesīut I am missing some type of models I do not find on any of this sites. Being super scared of men due to past experiences, but unfortunately, trapped in an elevator with you. I found a lot of models over the the time. It seems also possible to convert DAZ3D(?) to XNALARA and then to MMD, but I have not looked into this. Then I learned that XNALARA (xps) models could be converted (via Blender) to MMD and a lot more models became accessible. The 1.9 update bring loads of new features and character models to the already popular adult VR game, which should make supporters very content going into the holiday season. The Waifu simulator uses MMD models (pmx) and I therefore learned how I could import new models and how I could manipulate models (just basic stuff) in the PMX editor. To kick things off a good friend, Lewd Fraggy, released a highly anticipated update to Waifu Sex Simulator. I started some time ago to use the Waifu Sex Simulator with my VR glasses to create life like sex, gore or other scenes. Consequently, banks have lost about $8 billion to $10 billion in annual revenues to fintechs. The growth is underpinned by increased consumer and merchant awareness and adoption of point-of-sale financing solutions.Ībout 60 percent of consumers say they are likely to use POS financing over the next six to 12 months.įintechs are capturing almost all the value being created in POS financing because banks have been slow to respond. This is the only unsecured-lending asset class that has experienced high-double-digit growth through the COVID-19 crisis. POS financing’s expanding role in unsecured lendingĬredit originated at point of sale is projected to continue its growth from 7 percent of US unsecured lending balances in 2019 to about 13 to 15 percent of balances by 2023, according to data from McKinsey’s Consumer Lending Pools (Exhibit 1). The insights are based on McKinsey research, including McKinsey Consumer Lending Pools (a proprietary database covering granular market size and growth trends), the McKinsey POS Financing Consumer Survey and POS Financing Merchant Survey, and our recent experience with banks and merchants. It provides an overview of the market, details key trends and factors influencing growth, and offers ideas for market entry for banks and partnerships for merchants. This article seeks to give POS financing players as well as merchants the necessary insights to refine their strategies in the POS-financing arena. To avoid that outcome, US banks need to understand the landscape for POS financing and choose from among the emerging models. Banks that underestimate the threat may see continued loss in share and could lose out on participating in a growing value pool and gaining share among younger and new-to-credit customers, as banks in Australia and China did when facing a similar situation. In our view, only a few banks are responding fast enough and boldly enough to compete. Thus far, fintechs have taken the lead, to the point of diverting $8 billion to $10 billion in annual revenues away from banks, according to McKinsey’s Consumer Lending Pools data. This article was a collaborative effort by Puneet Dikshit, Diana Goldshtein, Blazej Karwowski, Udai Kaura, and Felicia Tan, representing views from McKinsey’s Financial Services Practice. Lepidus, Pontifex Maximus of Rome and the man who secured Caesar’s dictatorship. Marc Antony, Caesar’s loyal friend and most trusted lieutenant. Octavian, Caesar’s adoptive son and the heir to his legacy. The republic remains whole, but its soul is divided as three great men, the members of the Second Triumvirate, hold the future of Rome in the palms of their hands. The Imperator Augustus Campaign Pack is set in 42 BC during the chaotic aftermath of Caesar’s grisly murder. This campaign comes as part of Total War™: ROME II – Emperor Edition and is available as a free, automatic update to existing owners of Total War™: ROME II. The Imperator Augustus Campaign Pack is a new playable campaign for ROME II, which rivals the original ROME II Grand Campaign in both scope and scale. If you’re new to Total War: ROME II click the Total War Academy link to learn more: Ībout the Imperator Augustus Campaign Pack The Imperator Augustus Campaign Pack and all Emperor Edition updates are free, via automatic update, to all existing ROME II owners. In addition, a free DLC campaign pack ‘The Imperator Augustus’ is included, which follows the aftermath of Caesar’s demise. Emperor Edition is the definitive edition of ROME II, featuring an improved politics system, overhauled building chains, rebalanced battles and improved visuals in both campaign and battleĮmperor Edition contains all free feature updates since its release in 2013, which includes bug fixes, balancing, integration, touchscreen, and Mac compatibility. This was made possible by installing an Audio Control LCQ1 interface to the factory system. We knew we could achieve our goals without eliminating the factory Sync package and all other associated features. There was absolutely no reason to replace the radio in the dash. Since this was a Platinum Series F150, it came with the Ford Sync system and navigation. To solve this, we installed Hushmat Ultra Damping Padding in all four doors. A small rattle can make that really nice speaker completely lose it’s appeal. One issue that needs to be addressed in any type of vehicle where we install a nice stereo is the vibrations in the doors. Once the truck was all finished you would never have known the speakers had been touched just by looking at it. As you can see in the attached photos of the install, the tweeters and woofers fit in the original locations. We were looking for something that could handle real power and would deliver, and the C5’s exceeded expectations as usual. The speakers deliver a crisp clear sound even at much higher volume levels. This line of speakers from JL Audio is a force to be reckoned with. We installed JL C5 component speakers in the front factory locations, and C5 coax speakers in the rear. The first thing that we knew needed to be done was to install a better quality speaker package. We were honored to have the ability to bring the stereo of one such truck to a whole new level for a good customer of ours. Even with the upgraded factory Sony package, the stereo doesn’t give the “ Platinum” name any justice. The one big feature missing… is a good sounding stereo. Had to cut a notch for the seat belt bracket to fit.A 2011 F150 Platinum edition comes with more options than you might think, for a pickup truck. This is the before sound deadener and speaker install I had to trim about 1/4" off where the amp sits. Installed the amp and DSP on a piece of stained wood I had laying around. I'm still waiting on the rest of my orders to arrive, I'll post pictures and update soon. However it's still not where I want it to be. The sound is much improved in both the front and back seat. So far I've installed the speakers and sound deadener on all 4 doors, still running on the factory amp. I know I bought the ecoboost v6, no need to pretend there's a v8 under there. I'm putting sound deadener over the doors to block sounds, certainly don't need silly sounds playing from speakers. There's already a write up on this forum for that. Prior to this I did go ahead and cut the wires that play the V8 sound over the speakers. The goal is to retain factory look and function, and make it easy to put back to factory when I sell the truck. These are the connectors that plug into the door wire panels. I also bought a terminal tool to remove the connectors from the old connector, since I will keep using the Sony DSP to push the center channel. I wanted to use this instead of hacking up my old one. Motorcraft WPT1145, this is the connector that plugs into the Sony DSP. Infinity Reference 3002CFX for the center According to Crutchfield they don't fit this truck, but I can promise you they do.Īudison Bit Ten D to sum all the channels Also for the door speakers I bought 2 sets of Metra 72-5602 from Crutchfield. I used the supplied speaker adapter as well. Polk Audio DB652 6.5", also from Crutchfield. They came with the adapter brackets and it works great. So far I have.ĪudioFrog GS693 6x9's in the front door, bought from Crutchfield. The plan is to replace all speakers, add an amp, add a subwoofer, and have all the speakers except the center processed trough a DSP, and add sound deadener to the doors. So just going to start this small, then build on it. And if they are Sony systems most just upgrade the speakers. So I've seen a few posts on here about upgrading speakers, but most of them apply to the non-Sony systems. |
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